Abstract: Most individuals in the US spend a significant amount of time at work. Happiness at work becomes an important element of an individual’s overall happiness. Some findings suggest that job satisfaction can be determined by each person’s internal characteristics and not necessarily due to the employer. These internal characteristics may be related to culture. Miami is a city with a large Latino immigrant community. It was hypothesized that there is a significant difference between Latino immigrants and born-in-America individuals in their perception of happiness at work. In addition, it was also hypothesized that there is a significant correlation between the perception of happiness at work and the individual’s acculturation level. This research study utilized 100 respondents, who were selected via convenient quota sampling. The sample was divided into two groups according to their immigration status: Latino immigrants, and individuals who were born in America. Participants were given a questionnaire with 25 items intended to measure perception of happiness at work, acculturation level, and demographic information. A t-test and a correlation were used to test the hypotheses. However neither of these hypotheses were confirmed by the research results. These results suggest that Latino immigrants and American born individuals are more similar than different in terms of happiness at work.