Abstract: Plants based natural products have been widely used as curative agents for variety of ailments since immemorial time. In this study, powered plant material, leaves of Pongamia pinnata sequentially extracted using petroleum ether, ethyl acetate, ethanol, methanol and water and the extracts were concentrated to dryness by evaporating the solvent at 40oC. In vitro antibacterial activities of these extracts were studied by agar well diffusion method against gram positive Staphylococcus aureus and gram negative Escherichia coli. Streptomycin and different solvent extracts (petroleum ether, ethyl acetate, ethanol, methanol and water) were used as standard and control respectively. Phytochemical analysis also done to report the presence of biomolecules. This study demontratrated that the ethyl acetate extracts was higher antibacterial activity against tested bacterial pathogens than other extracts. All these extracts were able to inhibit the growth of S.aureus except aqueous extract. Phytochemical screening of the methanol, ethanol and aqueous plant leaf extracts revealed the presence of saponins and terpinods. The tannin was present in methanol extract and flavonoids was also present in methanol and ethanol extracts. The results also indicated the less antibacterial activity of different solvent extracts to standard, streptomycin and control also rather than didn’t reveal any antibacterial activity against the pathogens. This present study concluded that the antibacterial activity of various extracts of leaves of P.pinnata was carried in attempt to support the use by medicinal practitioner for the treatment of various diseases. However, further studies should be needed for the isolation and characterization of these active compounds.