Abstract: Nanosciences and microbiology are the research of extremely small objects on a nm scale. Tribolium Castaneum and Trogoderma Granarium isolates of various ages have been reported from the Lahore commodity market. For each of the two insects, the population had been acclimated to the laboratory. To maintain a homogenous population, pupa of the same age were collected during insect development and placed in separate plastic jars until adult emergence (2 weeks).