Abstract: The present study attempts to isolate and identify the number of endophytic fungal association in three species of bamboo leaves; Dendrocalamus sikkimensis (DS), Bambusa polymorpha (BOP) and Phyllostachys bumbusoides (PB). The endophytic fungi was isolated from the plant tissue following the protocol of Suryanarayanan et al., 1998. A total of 196 endophytic fungi were isolated and identified up to genus level. Among all the fungal isolates, Xylaria and Nigrospora sp. showed highest level of colonization frequency (i.e. 18%). Xylaria sp. was more dominant in BOP, whereas, DS and PB displayed deviation in terms of fungal dominance with sp2 (sterile) and Nigrospora sp. to be dominant respectively. The isolation of endophytic fungi from healthy mature leaves of host plant suggest that the fungi residing inside them is asymptomatic and may be beneficial to their host, as they are found to protect their host against domestic herbivores, pest and pathogens. The study opens the gate for further biological analysis of endophytic fungi associated with bamboo creating a genetic resources for future researchers.