Abstract: To check and analyze the effect of nickel sulphate toxicity on radish plant, a research was conducted in the old Botanical Garden at University of Agriculture, Faisalabad. In different concetrations of nickel sulphate solution (10, 20, 30 mM), Raphanussativus specie of radish (Mooli Day-40) was grown to study the morphological parameters like shoot and root length, shoot nad root weight, number of leaves, total carotenoids and chloropyll. Ni-stress remarkably reduced the growth attributes of radish plant. Results were reported after data collection and applying statistical anaylsis by using latest software packages. A noticeable drop in physiological and morphological attributes was recorded. However in biochemical attributes level of chlorophyll a and b was reduced while subsequent elevation was noticed in carotenoid concentration.