Abstract: Background to the Study: Human cytomegalovirus (HCMV) is a member of herpesvirus family. It is the most genetically complex viral pathogen of humans and now the commonest congenital form of infective neurological handicap recognized by the institute of medicine as the leading priority for the developed world in congenital infection. HCMV infection among women of reproductive age especially those that are pregnant can lead to congenital abnormalities and is often associated with serious complications such as microcephaly, mental retardation, deafness and spastic paralysis. This study was done to determine the seroprevalence rate of HCMV infection and its associated risk factors among women of reproductive age in three selected hospitals in Zaria metropolis, Kaduna State.