Abstract: An experiment was conducted during January 2015 to assess the present status of arsenic contamination and find out the relationship with soil properties and arsenic at Alamdanga upazila of Chuadanga district and Trishal upazila of Mymensingh district. Arsenic contamination and it`s relationship with soil properties (e.g. soil pH, total organic matter, total N, available P, available S, Exch. Ca, Exch. K and Exch. Na) of Alamdanga upazila and Trishal upazila were studied in the Soil Science laboratory at Bangladesh Institute of Nuclear Agriculture (BINA), Mymensingh. Arsenic contents of Alamdanga upazila ranged from 8.81 – 16.26 mg/kg and at Trishal upazila ranged from 6.58 – 9.16 mg/kg. Neutral acidic condition was observed at Alamdanga upazila (6.45 to 7.62) range where at Trishal upazila soil samples were slightly acidic in nature (6.38 to 6.69) range. Organic matter content in the soils of Alamdanga upazila ranged from 0.83 to 1.10%;