Abstract: The experiment was carried out at the Biochemistry laboratory and Mushroom Culture House (MCH), Department of Biochemistry, Sher-e-Bangla Agricultural University, Dhaka, during the month of June to November’ 2015 to investigate the performance of different sawdust on the yield of Oyster mushroom (Pleurotus ostreatus). Five different sawdust viz. mango tree (Mangifera indica), rain tree (Albiia saman), teak tree (Tectona grandis), mahogony tree (Swietenia mahagoni) and mixture of all four sawdust supplemented with 30 % wheat bran and 1 % CaCO3 as basal substrate were selected for determine the yield of oyster mushroom (Pleurotus ostreatus). The highest weight of individual fruiting body (5.60 g), dry yield (35.15 g), biological yield (368.18 g) and economic yield (360.68 g) were also found in mango tree sawdust. Therefore, it can be concluded that mango tree sawdust supplemented with 30 % wheat bran can be further used as a better substrate for maximum yield of oyster mushroom (Pleurotus ostreatus) among following substrate.