Abstract: Handloom industry plays a vital role in the socio-cultural and economic life of the people. It is dependent on manual labor and here the major contribution comes from women. The present study was conducted on women workers, who perform the activities in handloom industry of Lakhimpur district of Assam to find out the musculo-skeletal problems and perceived joint discomfort of their body due to uncomfortable working postures. Various parameters observed were weight, height, angle of deviation, time and body mass index (BMI).A five point scale given by Vergese et al (1995) was used to record the musculo- skeletal problems and perceived joint discomfort. A sample of 40 women workers of Lakhimpur districtAssam was selected for the study through multistage random sampling. Statisticalanalysis of the data are employed through frequency, percentage, mean, standard deviation, critical difference and co-efficient of correlation.Maximum joint discomfort was found in the waist(4.02)followed by finger (3.85),wrist (3.55),elbow (3.47), shoulder (3.45), and in knee(2.72) respectively while performing different activities in handloom. About 47.5% respondents perceived very severe pain in back. Less than 45% respondents made complaint of severe pain in low back. The 57.6% respondents made complain of moderate pain in leg followed by mild pain in fingers (32.5%) and knee (12.5%).