Abstract: The rise in global oil demand along with current environmental crisis; global warming and climate mitigation has been a serious issue. Thus, the need for an alternative fuel from renewable resources that promotes sustainability is vital to replace our dependence on fossil fuel. High sugar content in Oil Palm Frond Juice (OPFJ) indicates its potential as a feedstock in ethanol fermentation. Two strains of Saccharomyces cerevisiae; SA79 and HC10 are screened for high fermentation efficiency. Percentage yield bioethanol revealed that S. cerevisiae HC10 (55.72%) was proven a better strain than S. cerevisiae SA79 (43.19%). A growth profile of S. cerevisiae HC10 was performed in 60 h time period with 6 h interval sampling time. Specific growth rate (µ) and doubling time (td) of 0.037h-1 and 18.7h, respectively shows that S. cerevisiae HC10 fermentation of OPFJ is industrially applicable.