Abstract: Triglycerides are stored lipids with high calorific value, and so may be the preferred choice of fuel during starvation, which may be partially utilized and also may be a simultaneously synthesized along the degradation, probably to balance the lipid over load and also to prevent lipotoxicity of cells caused due to accumulation of FFA(free fatty acids). There may not be any absolute depletion of lipids, during starvation as lipid is considered to be the element constant. To study and understand the relevance of triglycerides during starvation we selected, Anabas, testudineus, a sturdy fish from coastal waters of Andhra Pradesh (INDIA) and subjected it to brief (15days) and prolonged fasting (60days).Triglyceride concentration was estimated by the method of Raghuramulu et.al (1983).Six non adipose tissues, like liver, kidney, brain accessory respiratory organ, pectoral and lateral line muscle were chosen for the study. There was an overall upsurge observed in the triglyceride levels. During the short term fasting stress, the increase was found to be a significant one in tissues such as liver (P<0.01), kidney,(P<0.001) brain,(P<0.001) accessory respiratory organ(P<0.001) and lateral line muscle(P<0.001). Pectoral muscle (NS) however showed an insignificant rise. Long term starvation results were different from that of the short term. Tissues such as kidney(P<0.05) and brain(P<0.05) showed a significant rise and other non adipose tissues such as kidney, pectoral and lateral line muscle, all showed an insignificant increase. Accessory respiratory organ did not show any change in the triglyceride content. Anabas, adapted well to the starvation stress and survived all through the experimental period.We suggests that the triglyceride synthesis and accumulation during fasting may be used as an index to describe starvation status of anabas.