Abstract: Now a Days increase atmospheric temperature is the matter of great discussing issues for the environmentalists to gain more relevant information on tolerance of the organism to the temperature. The sensitivity of fishes exposed to the pesticides as well as the changed biological factors. Temperature as an abiotic factor showed influence on the physiochemical parameter of all living organism. The present study was carried to evaluate the impact of different temperature range (35˚C and15˚C) on the fresh water fish Channa punctatus. Alteration of the rising temperature observed on the opercular beats and respiratory rate showed increased trend, while the average opercular movements per minute at below room temperature showed decreased as compared to normal values.At 35˚C temperature the respiratory rate were increased as compared to normal values, while at 15˚C temperature treatment the respiratory rate of Channa punctatus was found to be decreased.