Abstract: Sadagura a special smokeless tobacco preparation is being very popular in southern part of Assam, North -East India. The study is the first report on the genotoxic effect associated with the sadagura (SG) with or without areca nut (AN) extract in vivo in albino mice. Analysis of chromosome aberration (CA), micronucleus (MN) were performed in the bone marrow cells and sperm head abnormality (SHA) assay, total sperm count (TSC) were done in germ cells compared with control.