Abstract: In the present study, the isolate fungus from infected insects of mini-farm was studied of cultural, microscopic morphology, antifungal susceptibility test and the growth condition concerned with biomass and spore counting. The isolate fungus was close to Metarhiziumspecies that confirmed based on cultural and microscopic morphology. The biomass of fungi was studied that the highest dry weight was 0.0667 at 5 days. And the logarithmic number of spore per milliliter was increased till 9.6 at 5 days. The two growth curves have short time stationary phases and they were gradually increased before around 5 days. Ketoconazole was the highest antifungal effect on the isolate strain of Metarhizium sp.