Abstract: Milk easily undergoes microbial contamination and spoilage because it is a rich biological fluid which contains all necessary nutrients for animals. This study was carried out to evaluate the microbiological quality and safety of cow milk at different sampling points; selected chilling centers, milk transportation vessels and farmers in Anuradhapura district of Sri Lanka. A total of 11 chilling centers were selected for the study and classified them as low and high risk centers. Total 80 samples were tested for total bacterial count/standard plate count (SPC) and total coliform count (TCC) and analyzed by using Poisson Regression in GENMOD Procedure in SAS. Descriptive statistics of standard plate count and total coliform count data were obtained using Minitab 17.