Abstract: Trees excluded from the forest and other wooded lands are defined as “Trees outside forests (TOF)”. TOF are mostly located on farmlands and built-up areas of rural and urban regions. Remote sensing with its unique capability of synoptic viewing, real time and repetitive nature offers a potential tool for monitoring and evaluation of Earth’s natural resources. Forestry is one of the most important disciplines in which remote sensing technology is being used over a considerably long time period from the early stage of its development. Remote sensing technology has been successfully employed in various studies like forest inventory, monitoring of forest cover changes, forest damage assessment. In the present research a study has been undertaken to develop an operational method for mapping and monitoring trees outside of forests by using the freely available moderate resolution satellite remote sensing data in the Barisal Division of Bangladesh excluding the Jhalokati District. Landsat 8 OLI (Operational Land Imager) data of 2016 and Landsat ETM+ (Extended Thematic Mapper) data of the year 2000 have been used for this purpose. A comparison of TOF has also been done in the five Upazillas of Barguna District between the year 2000 and 2016. Driving forces behind the changes of TOF has also been investigated through GPS (Global Positioning System) based ground verification and interview with the people living in the locality.