Abstract: Microorganism may adapt with environmental stresses by altering metabolisms and structures of their physiology. Temperature is a major environmental factor that can be used to inhibit microorganisms. However, sublethal temperature stress will not kill microorganism effectively and yet microorganisms are injured. The objectives of the present study were to determine the culturability and percentage injury of Escherichia coli ATCC 25922 grown in the laboratory medium when shifting from 37°C to sublethal temperature stresses temperatures of 45°C, 40°C and 20°C at log and stationary phases. Initially, the E. coli culture was grown in Tryptic Soy Broth at 37°C for 6 hours and 18 hours to reach log phase and stationary phase, respectively before the culture was transferred to three sublethal temperature stresses and culturability were determined by using two-plating systems;