Abstract: Use of probiotic bacteria in aquaculture has tremendous scope and the studies of the application of probiotics in aquaculture have a glorious future. The present study was aimed at the studies on the probiotic characters of bacteria which were isolated from shrimp cultured ponds. Initially 26 soil samples were collected from dried shrimp cultured ponds; bacteria were isolated by serial dilution. 10 samples were able to produce prominent colonies which were further subjected for repeated sub culturing for pure cultures on nutrient agar medium. Among 10 isolates six were Gram positive, and four of them were Gram negative.