Abstract: This study aims to determine performance of Bacillus subtilis probiotic with addition of purple sweet potato and cassava prebiotics on immune response of tiger shrimp. The research is carried out at the Mini Hatchery of the Faculty of Marine and Fishery Sciences and the Laboratory of Fish Parasites and Diseases, Department of Fisheries, Faculty of Marine and Fishery Sciences, Hasanuddin University. The stages in this study consisted of: (1). Prebiotic preparation (of purple sweet potato and cassava), (2). Probitic preparation, and (3). Vibrio harveyi challange test. This research uses a completely randomized design with 4 treatments and 3 replications.