Abstract: This study sought to find out factors that affect the performance of pre-service teachers in college algebra in some selected Colleges of Education in the Bono Region of Ghana. A quantitative survey design was employed in this study. The study was conducted in two Colleges of Education and sixty – six (66) second-year pre-service teachers offering 4-year Bachelor of Education(B.Ed.) Programmes in both Primary and Junior High School ( JHS) education randomly selected. A five- point Likert scale questionnaire was used to collect data on the three factors (students’ attitude, motivation, and lesson presentation). Twenty (20) items questionnaire was administered to the pre-service teachers to collect data. The data were analyzed and the descriptive statistics involving means and standard deviation as well as correlation, regression, and paired samples t-test were used to address the research questions. The findings revealed that the poor performance of students in college algebra in colleges of education in Ghana was caused by students negative attitude, motivation and lesson presentation. It is, therefore, recommended that these negative attitude needs to be corrected by instilling in students the values in mathematics. Teachers teaching college algebra should be innovative and use the appropriate technique to teach the concepts in college algebra. The government and stakeholders in teacher education should provide necessary materials and other resources in colleges of education to harness the potentials of pre-service teachers.