Abstract: Information on positive living as well as sexual and reproductive health (SRH) is essential for HIV infected adolescents on anti-retroviral therapy (ART). There was very little intervention study among them and effects of intervention have not studied yet. A quasi-experimental study encompassing baseline assessment, intervention and post-intervention assessment after one month was done by applying a mixed-methods design at Mandalay and Magway in 2019. Current study aims to assess the effectiveness of the intervention in terms of knowledge and attitude on SRH and positive living. Intervention package included aspects of positive living incorporated with SRH information which were conducted in group sessions including body mapping, case scenarios and discussions.