This study was planned to identifying the Microorganisms are present in oral cavity as normal microflora. However the fact that they become harmful due to some oral infections made us to investigate the inhibitory effect of orange juice extract on them. Total 20 samples were collected. Samples were taken from patients suffering from throat infection. On the basis of morphological variations 6 strains were selected and were tested for their sensitivity against orange juice extract. Some strains are resistant but 3 strains were sensitive against orange juice extract. Aqueous layer of juice extract proved to be more effective in their antibacterial activity on studied strains as compared to methanolic ones. Minimum inhibitory concentration (MIC) values of these extracts were also determined. Moreover, these fruit extracts also tested for their antibacterial activity against clinical isolates and proved to have significant inhibitory effect on growth of these isolated bacteria, providing an alternative to treat various infections caused by these antibiotic resistant strains. Hence this study also enhances the nutrition value of orange juice because orange juice provides the resistance against some kind of oral bacteria.