Abstract: Silver nanoparticles (AgNPs) have received considerable attention due to their attractive physical, chemical, antimicrobial, anti-inflammatory and wound healing properties. This paper reports simple, cost effective and eco-friendly methods for the preparation of AgNPs using silver nitrate solution as the metal precursor using leaf extracts of Ocimum tenuiflorum, Azadirachta indica and Plectranthus amboinicus. The AgNPs obtained were characterized by XRD to analyze the particle size. The XRD patterns confirmed the purity, phase composition and nature of the synthesized nanoparticle. UV-Vis and IR spectroscopy was used as a tool to characterize AgNPs. The study also revealed that AgNPs synthesised from all the medicinal plant extracts had inhibitory activity on the gram positive and negative human pathogenic bacteriae Bacillus and Klebsiella. The broad spectrum of bioactivity of AgNPs makes them promising agents not only to fight infections, but in many other biomedical areas.