Abstract: This research was carried out to evaluate the phytochemical and antibacterial effect of ethanol extract of Vitellaria paradoxa seed on Staphylococcus aureus and Candida albicans. The research was conducted at Microbiology laboratory of Federal college of Forestry, Jos.V. paradoxa seeds were collected from Bida, Niger State, Nigeria. Clinical isolates of S.aureusand Candida albicanswere obtained from the stock culture of Microbiology Diagnostic Laboratory, N.V.R.I., Vom, Plateau State. Susceptibility testing was carried out using agar well method.100, 50 and 25gm/mlconcentrations of the extract was used to determine zone of inhibition and water served as control. Minimum bactericidal concentration (MBC) and minimum fungicidal concentration (MFC) was conducted using tube method at double fold dilution. MIC and MBC of the extract were tested at concentrations of 25, 12.5, 6.25, 3.125, 1.5625 and 0.7812mg/ml. Ciprofloxacin 20mg/mlserved as positive control and replicated three time.