Abstract: The present investigation was undertaken with the main objective to determine studies on seedborne fungi of soybean were conducted at DSST and Plant Pathology, College of Agriculture, Rajendranagar, Hyderabad. A total of 120 representative soybean seed samples (cv. JS - 335) were collected from major soybean growing districts of Andhra Pradesh viz., Adilabad (60 samples) and Nizamabad (60 samples) during Rainy season 2012. Per cent total incidence of seed mycoflora in Nizamabad and Adilabad districts ranged from 30 to 49.2 % and 23.6 to 45.0 % by blotter method, 14.8 to 28.1% and 11.6 to 22.1% by 2, 4 - D blotter method, 11.8 to 19.3 % and 9.5 to 16.2 % by deep freeze blotter method, 13.1 to 37% and 15.4 to 26.4 % by agar plate method, respectively. Out of nine fungal species recorded, Macrophomina phaseolina was found predominant in the samples analysed from two districts (8.5 to 28.5 %), while the occurrence of Cladosporium sp. (0.3 to 0.5%) was least. Seed transmission of M. phaseolina in apparently healthy soybean seeds (cv. JS 335) was 6 % and 8 % and in artificially inoculated soybean seeds (38.5 % and 49 %) and in naturally infected soybean seeds (32 % and 43.1 %). Germination in the above seed samples ranged from 75% to 72%, 55% to 46% and 59.3 % to 50.5 % in test tube water agar method (in vitro) and in glasshouse conditions.