Abstract: Every individual in the world can be, identified at the molecular level on the basis of an extremely high level of polymorphism in the sequence of his or her DNA, which he or she inherits from his or her biological parents and is Identical in every cell of the body. DNA fingerprinting, as this technique of identification is called, can confirm with certainty the parentage of an individual. The application of DNA profiling in the criminal justice system is an important issue in criminal investigators today. The technology is changing rapidly and several new techniques are becoming available. DNA profiling has been described as a powerful breakthrough in forensic science. The Forensic Use of DNA Profiling is a major contribution to a technology which can help not only in including the culprit but also to exclude the innocent. In this article an attempt is made to elaborate the changing scenario of the technology in the recent years as well as to present the some real cases where different variants of the DNA fingerprinting technology were successfully applied in solving the criminal cases in our laboratory.