Abstract: Studies on coastal biodiversity of agro-ecosystems in Guyana are limited. The agriculture sector contributes significantly to Guyana’s economic growth and most of these agricultural activities are concentrated on the Low Coastal Plain of Guyana. This study assessed the impacts of agricultural activities mainly rice and sugarcane farming on freshwater fish diversity within Mahaicony and Enmore areas, located in Region 4 of Guyana’s Coastal Plain. It was hypothesized that agricultural activities would adversely impact fish species diversity within the disturbed sites by lowering species richness and species evenness compared with undisturbed sites.