Abstract: Despite many efforts in preventing the transmission of pathogens in health care facilities, little has been done related to the potential of contamination and transmission of microorganisms between users on health services. Fungal elements may be important sources of contamination in physiotherapy ambulatory. The aim of this research is to identify fungal elements on physiotherapy clinic stretchers. Methodology: sterile swabs embedded in sterile saline solution were used to collect the material, which were rolled in zigzag movements over the entire surface of 60 stretchers from the Clinic of the UNIABEU University Center Physiotherapy School. The material was seeded in Petri dishes containing the media Sabouraud dextrose agar and Mycosel culture. The plates were sealed with adhesive tape and maintained at room temperature (±30°C). Colonies of filamentous fungi in microcultures were identified by cultural and morphological characters and yeasturiform fungi by Gram staining and biochemical tests.