Abstract: The aim of this study was to determine the incidence of Toxoplasmosis in pregnant women and to assess the possible risk factor associated with pregnant women living at Najaf Governorate -Iraq. This study was carried out from January 2016 until March2016. Through the serodiagnosis of (114) pregnant women,the result shown that the (54) pregnant womenwere parasitic for Toxoplasmosis giving an overall infection rate (47.4%).The highest rate of infection with toxoplasmosis observed in the age group≤ 25 years with the percentage of (59.3%),and in the residence the results showed that the percentage of infection with T.gondii was higher in urban areas (83.3%), while in level of education the result appear that the highest incidence of infection with T.gondii occurs in primary school graduated with the percentage (38.9%), The statistical analysis of the results shown the significant decrease(≤ 0.05) in T.gondii infection with sufficient monthly income compared to the insufficient monthly income .