Abstract: Visnagar city is located in north Gujarat (72 30 n and 73 30 n 23 0 e and 23 35 e). Visnagar taluka is popularly known as ‘Shikshan Nagari’ and also known as Copper city. The climate of visnagar is tropical arid to marginal semi-arid. It is strongly periodic and seasonal. There are many fresh water bodies are situated at and around Visnagar taluka. The present study deals with the physico-chemical status of two lakes, deliya lake and Malap lake, under biotic stress”. Deliya lake is natural fresh water body having 19 hector area & circular in shape. It is located between latitude230 41’ 60’’N longitude720 32’ 60’’ E. it is oldest lake of visnagar. It is also known as hanuman temple talav. Constructed before 10’th century. Another historical lake is Malap lake , is also situated near Visnagar. Malap lake is natural fresh water body having 4 hector area. These water bodies has dense growth of algae and planktons in its. Physico-chemical status of two lakes belongs to Visnagar Taluka were studied in year January to June 2011. Both the lakes are biotically affected by various anthropogenic activities. In the present study water characteristics of two lakes have been compared the water quality. Different Parameters carried out like temperature, pH, Fluoride, COD, BOD, Phosphate, Sodium, Chloride, Alkalinity, Total Hardness, Calcium, DO and TDS. The result indicates that the both lakes are in polluted condition. It is evident that Malap Lake was found to be more polluted in compare to Deliya Lake. Mittal & Sengar (1990) investigated phytoplankton diversity in relation to certain physico-chemical characteristics and observed direct correlation with conductivity, dissolved solids, suspended solids, turbidity, D.O. and B.O.D. Tripathi and Pandey (1990) observed higher value of total hardness and stated that it may be due to polluted water of the ponds. Various physico-chemical parameter like Different Parameters analyzed like pH, Fluoride, COD, BOD, Ch