Abstract: Cervical cancer caused by Human Papilloma Virus (HPV) has claimed the lives of many women worldwide. The study aimed at determining the prevalence and associated risk factors of HPV infection among pregnant women attending outpatient clinic in General Hospital, Lapai Niger State, Nigeria. A total of one hundred and eighty four (184) pregnant women were enrolled, blood samples were subjected to ELISA screening for HPV and further screened for HIV, Hepatitis B and C using determine and HBsAg/HCV Rapid test strip methods. While ABO blood grouping reagents were used to detect blood grouped types. Questionnaires were used to obtained data from participants and analyzed statistically.