Abstract: Chronic Hepatitis C is the most common cause of liver disease and liver cirrhosis and it is due to common spreading of HCV by liver transplantation in the United State (U.S) Australia and most of Europe. Approximately 170 million people are affected by HCV worldwide and 3% are in global population. HCV is the most common chronic blood born infection in the U.S and its involved 40% in chronic cases of liver disorders (Wasley & Alter, 2000)). HCV was first time isolated from a person who has not affected with hepatitis A and hepatitis B in 1989 (QL, et al., 1989). Shortly after the cloning of HCV this new-found virus was discovered to be the cause of approximately 90% of non-A and non-B hepatitis in the U.S. (Lauer & Bruce, 2001)