Abstract: Ultrasonic velocity, density and viscosity of binary liquid mixtures of Dimethyl sulphoxide (DMSO) (0, 10, 20, 30, 40, 50, 60, 70, 80, 90 and 100 volume percent) in 2-Propanol have been measured at various temperatures. The acoustical parameters such as adiabatic compressibility (), acoustic impedance (Z), free length (Lf), relaxation time (), free volume (Vf), available volume (Va), internal pressure (i), Gibbs free energy (G), Molar sound velocity or Rao’s constant (R), absorption coefficient (α/f2) and enthalpy (H) have been obtained from the experimental data of the liquid mixtures with a view to investigate the exact nature of the molecular interactions. The Excess values of adiabatic compressibility (E), acoustic impedance (ZE), free length (LfE), free volume (VfE), available volume (VaE) and internal pressure (πiE) have also been calculated and found to be useful in estimating the strength of molecular interactions in the mixture.