Abstract: A pot experiment was conducted in glass house conditions for 120 days to study the effects of phosphorus solubilizing Gram-positive bacteria on plant growth, nutrient uptake and yield of cowpea (Vigna unguculata). Among all twelve bacterial treatments, the strain Bacillus spp (B3) has shown the greatest shoot length 67.70 cm, root length 60.5 cm and total dry weight 4.57 g plant-1 after 45 days of sowing. Similarly, in the same treatment the shoot length, root length and total dry weight were found 162.36 cm, 60.5 cm and 128.49 g plant-1, respectively at harvesting and total yield was obtained 23.34 g plant-1, which was significantly greater than all other treatments and uninoculated control treatments containing single super phosphate (SSP) and rock phosphate (RP) as phosphorus sources (7.93 g plant-1 and 3.81 g plant-1 respectively). The strains Bacillus thurengensis (T1 and T6) also have indicated a significant increase in above parameters i.e root length, shoot length, total dry weight and yield. In Bacillus thurengensis (T1 and T6) the total yield was observed 19.50 g plant-1 and 18.03 g plant-1 respectively. Besides these above strains a significant increase in growth and yield of cowpea plants was observed in all the Gram–positive phosphorus solubilizing bacteria inoculation compared to uninoculated control.