The present study was conducted with the aim to form an inclusion complex of a toxic carcinogenic mutagenic agent Ethidium-bromide (EtBr) with the host molecule β-cyclodextrin (β-CD). The spectroscopy study of this inclusion complex in absence and presence of β-CD was carried out by Nuclear magnetic resonance (NMR). The binding constant and stoichiometry of the complexes were determined by Scott’s method. The inclusion complex of EtBr with β-CD was further confirmed by 2D 1H-1H NMR and further by molecular docking technique. The binding constant and stoichiometry ratio were found to be 301 M-1 and 1:1, respectively which indicate the successful formation of the inclusion complex between EtBr and β-CD.
Ranjna Jadon, Dinesh Sharma, Brijesh Sharma (2019); Spectroscopic Study of Inclusion Complex of the Ethidium Bromide with Β-Cyclodextrin in Solution NMR; International Journal of Scientific and Research Publications (IJSRP)
9(7) (ISSN: 2250-3153), DOI: http://dx.doi.org/10.29322/IJSRP.9.07.2019.p91108