Abstract: The famous fruit, Citrus reticulata Blanco was selected for chemical analysis and collected from the Pyin Oo Lwin Township, Mandalay Region in Myanmar. The preliminary detection of phytochemical compounds present in the sample was carried out by standard procedures. The sample gave rise to positive results for alkaloid, flavonoid, glycoside, polyphenol, tannin, reducing sugar and terpene respectively. The antimicrobial activities of sample in various solvent systems were determined by agar well diffusion method on six selected microorganisms such as Bacillus subtilis, Staphylococcus aureus, Pseudomonas aeruginosa, Bacillus pumilus, Candida albicans, and E. coli. The main objective of essential oil was extracted from the peel of Citrus reticulata Blanco by steam distillation method. The yield of essential was found to be 0.4960% based on the sample weight. Moreover, the extracted essential oil was analyzed by Gas Chromatography Mass (GC-MS)