Abstract: In this research work, the tube well water samples (1, 2, 3 and 4) were collected from Kanthayar Ward, Chanmyathazi Township, Mandalay on August, 2018 to determine the quality of groundwater. The physical and chemical properties of collected water samples such as color, pH, total dissolved solid (TDS), total hardness, total alkalinity, calcium, magnesium, sulphate and chloride were determined. Organic pollutant parameters such as DO (dissolved oxygen), BOD (biochemical oxygen demand) and COD (chemical oxygen demand) were also investigated. The content of heavy metals such as arsenic, lead and cadmium were determined by using atomic absorption spectrophotometer (AAS). The bacteriological examination of all water samples were investigated. The collected water samples from site 2 was treated by using rice-husk charcoal, sand and sponge as adsorbents. The physicochemical properties and heavy metals of tube well water sample before and after treatment were also determined. The present study provides information on the quality of tube well water from collected area in Mandalay Region.