Abstract: The genus, Trichoderma and its metabolites were meant for antimicrobial activity against the microbial strain and thus it is considered to be natural antimicrobial agents. Silver is also known as an antimicrobial agent and is utilized in several antimicrobials and medications. In the present investigation, the nano-particles were prepared both of Trichoderma and Ag+ separately and further after fusing Trichoderma and Ag+. Trichoderma harzianum secretes secondary metabolites which act as a capping and reducing agent. The biosynthesized silver nanoparticles (AgNPs) were characterized by UV–Vis spectroscopy and Transmission electron microscopy (TEM). UV–Vis spectra of silver and Trichoderma filtrate showed absorption spectra at 450 nm and 430 nm respectively while fused nanoparticles showed absorption spectra at 415 nm corresponding to the surface plasmon resonance of silver nanoparticles.