Abstract: The present communication deals with description of two ciliate species Anoplophrya chkrawartii and Anoplophrya krishnamurthii n.sp. isolated from the intestine of Perionyx excavatus and Pheretima posthuma collected respectively from different localities of Jalna district. Anoplophrya chkrawartii redescribed here which is previously described by Lalpotu as new species (1979) in Marathwada region. A new species (Anoplophrya krishnamurthii) differs from previously described species in having elongated macronucleus with irregular outlines. Micronucleus is small, elongated, somewhat banana shaped, 2 rows of contractile vacuoles, 2 vacuoles in each row and it measuring about 25.63-39.6 µm in length and width from 16.2-20.97 µm.