Abstract: Red wine is a complex fluid. It contains water, sugars, acids, alcohols, and a wide range of phenolic compounds. Red wine contains a number of biologically active compounds with beneficial effects on human health. The resveratrol is commonly found in food and drinks, including Red wine and grapes. Many studies have been documented towards health benefits of red wine consumption, including anti-oxidative, anti-carcinogenic, anti-inflammatory and anti-cardiovascular and antibacterial properties. Therefore, we evaluated the antimicrobial activity of red wine against multi drug resistant bacteria. The aim of the present study was to determine the antimicrobial activity of Red wine against 30 Multi Drug Resistant pathogenic strains of Staphylococcus aureus, β haemolytic streptococci, Escherichia coli and Klebsiella pneumoniae was studied using the agar well diffusion method.