Abstract: Glucoamylases are important enzymes that allow the hydrolysis of starch and related polymers to glucose. They can be obtained from microbial as well as other sources. Studies were conducted with two fungal isolates, Aspergillus niger MENA1E and Rhizopus MENACO11A obtained locally from infected plant materials to assess their potential in producing glucoamylase. The organisms were grown on four substrates: wheat bran, rice bran, groundnut pod and maize bran. Glucoamylase was produced by both organisms on all four substrates. The highest glucoamylase activities of 2.0 U and 1.99 U respectively for Aspergillus niger MENA1E and Rhizopus MENACO11A were recorded after 48 hours on wheat bran. Significant activities of the enzyme were also produced on the other wastes studied in this report.