Abstract: This research paper describes the automated toll collection system for toll gate based on RFID technology. Most of the toll collection systems commonly used in Myanmar is manual transaction. Nowadays, streams of traffic are increased and toll gate on highways are congested. It will cause the traffic jam and waste time. The objective of this journal paper is to transform manual transaction to automated toll collection with the help of RFID technology. There are three portions in toll collection system. They are RFID system, balance deduction system in host computer and toll gate control system.