Abstract: A study was conducted to investigate the effect of Chitosan on seedling growth and grafting success of Jack Fruit (Artocarpus heterophyllus) variety Father Long. Chitosan was applied as a seed treatment and seedling spray to root stock before grafting. It was used alone or combines with fungicide (Mancozeb 70 WP) and Albert’s solution i.e. commercial formulation of macro and micro nutrient mixture. Results indicated that spraying of Albert’s solution (2g l-1) was the best treatment for higher growth of seedlings. Seed treatment with chitosan (20 g l-1 for 90 min.) or Mancozeb (2 g l-1 for 90 min.) and foliar spraying of Albert’s solution showed higher dry matter accumulation of seedling compared to other treatments.