Abstract: Anthocyanins are phytochemicals belonging to class of flavonoids and polyphenolic molecules. In the current investigation, extraction of Anthocyanin pigments from beetroot peel and red cabbage leaves by cold maceration method was more effective expressing 4.2% yield when compared to Soxhlet method of 2% yield. Various methods of fractionation were implied namely column chromatography, analytical thin layered chromatography and preparative thin layered chromatography.