Abstract: Biodiversity is an important gift of nature that provides all basic requirements for human existence. But due to modern development of human races nature is under great threat. Since time immemorial plants have played an important role in human civilization. It has been observed that large numbers of plants are being used for the worshiping of gods and goddesses by different indigenous communities, which serve as a useful tool for conservation of plants. Hinduism has been called the oldest religion in the world and dominant in Indian subcontinent. The present paper analyzed different plant species that are used in worshiping of gods and goddesses in Hindu tradition in two districts of Assam viz. Nalbari and Sonitpur. A total of 54 species under 51 genera and 30 families were recorded during the study. Among these, Cynodon dactylon, Aegle marmelos, Ocimum sanctum, Piper betle, etc. are exclusively used in all worship. Prasad is one of the most important elements of all worship which is prepared with different varieties of fruits and cereals. Vigna radiate, Cicer arietinum, Mangifera indica, Musa sp., Saccharum officinarum, Cocos nucifera, Zingiber officinale, etc. are some important species used for the preparation of Prasad. In fact, the Prasad are rich in nutrients and considered very healthy.