Abstract: Present study conducted on coronary heart disease patients. plasma homocysteine concentrations and relationships to vitamin B9 that serve as coenzymes in homocysteine metabolism. Results showed that homocysteine exhibited strong inverse association with plasma folate. plasma homocysteine level varied from 6.8 to 18.7µmol/L with mean as 12.92±3.61µmol/l in normal control male subject. The mean plasma homocysteine level was increased to 18.89±2.24µmol/L with a range of 14.0 to 21.51µmol/L in normal control males subjects aged between 46-70 year ,increase in the homocysteine level was statistically significant as compared to that of normal subjects above stated as evident by P-value (P<0.001). The mean plasma Homocysteine level was found to be 23.48±1.62µmol/L with a range of 20.64 to 25.54µmol/L in CHD male subjects aged between (25 to 45 years).The increase Plasma homocysteine concentration in CHD patients was statistically significant as compared to normal control more subjects with age difference as evident by P-Value (P<0.001) .The increase was statistically highly significant as compared to control group. while it ranged from 6.80 to 21.51µmol/L as evident by P-value (P<0.001). CHD.The mean serum folic acid (vit.B9) level was found to be 6.98±1.54ng./ml with a range of 4.82 to 9.12 ng/ml in normal control male subjects aged between 25 to 45 years.Plasma homocysteine and vitamin B9 was estimated by high performance liquid chromatogrphy. Estimation of homocysteine and serum vitamin B9 is reliable, economic and sensitive and it can be used in proper management of chronic complications of coronary heart disease.Hplc grade kits.