Abstract: The leaf extracts of Commelina benghalensis L were subjected to phytochemical and antimicrobial analysis. Chloroform extract showed the best results. Presence of tannin, favonoid, phenol, carbohydrate and volatile oil were observed in the chloroform extract which showed the maximum activity against the pathogenic bacterial strains Escherechia coli, Staphylococcus aureus, Salmonella typhi and fungal strain Candida albicans with 7mm, 1mm, 3mm and 4mm of inhibition zone respectively compared to hexane and methanolic extract which showed the least activity.