This experiment was carried out to determine the tolerance level of winter buds according to positions in Karaerik cv. grape cultivar grown in Erzincan province during the winter colds that occurred in 2013/14 and 2014/15 years. For this purpose, frost tolerance levels of the first 4 buds of one-year old shoot and their damaged varability rates by exposure to low winter temperatures have been detected with binocular microscope and lipid peroxidation (MDA) analysis. The winter buds at the 2nd and 3rd shoot were found to be most sensitive to low temperatures with average damage rates of 26.5% and 33.5%, respectively during the years of the research. Additionally, the winter buds at the 1st and 4th nodes according to positions were found to be the most tolerant buds with average damage rates of 18.5% and 19.5%, respectively. On the other hand, the winter buds found on the 2nd and 3rd nodes according to positions were found to be the most sensitive buds with average MDA content of 3.72 and 3.74 nmol/ml respectively. For all that the winter buds in the 1st and 4th nodes according to positions were identified as the least injured buds with average MDA content of 2.92 and 3.12 nmol/ml respectively. Therefore, in pruning this is recommended to reduce yield losses by making it from node position 4th after the severe winter cold. In this way, with standardization of pruning levels for Karaerik grape cultivar can be improved yield and quality.