Abstract: The number of metabolic syndrome is increasing, necessity an assessment of knowledge to avoid this population growing. The aims of this study were to develop and validate Malay questionnaire on knowledge of prevention and risk on metabolic syndrome. Methodology: Cross sectional study was done among 200 samples from three villages in Bachok, Kelantan by face-to-face interview. Twenty-eight items were developed by a group of public health experts. To assess test retest reliability, 62 from all respondents were re-interviewed for the second time after two weeks interval. Result: Respondents were aged between 18 to 84 years old. Majority of the subjects were Malay (98%) and female (89%). Two items from the original questionnaire were deleted due to low correlations and after being judge unduly influence item. The factor loading using principal component analysis with varimax rotation was ranged from 0.41 to 0.84. Internal consistency of the components using cronbach’s alpha was ranged from 0.403 to 0.809. Intra Class Correlation (ICC) test was performed for test-retest reliability with the coefficient at 0.95. Conclusion:An acceptable questionnaire was developed for measuringthe knowledge on prevention and risk of metabolic syndrome. The feasibility was present and the internal consistency and ICC were excellent. It can be used to explore the baseline of knowledge of metabolic syndrome before tailoring anyhealth educationor promotion for preventing metabolic syndrome in Malaysia.