Abstract: The present study was conducted for detection of leaf curl begomovirus disease of sunflower (Helianthus annuus L.) in Tobacco (Nicotiana benthamiana). In the present study tobacco seedlings were inoculated with leaf curl virus of sunflower under laboratory through insect vector whitefly (Bemisia tabaci), The characteristic symptoms were observed 10-15 days after inoculation, further all inoculated samples were retested (3-4 weeks after inoculation) by molecular based Polymerse chain reaction diagnosis for the presence of virus. The results revealed that the causal virus of the disease was successfully transmitted from sunflower to sunflower (Helianthus annuus) and on Tobacco (Nicotiana tabacum L) in a short incubation period and also confirmed the presence of coat protein (CP) of leaf curl begomovirus in virus inoculated hosts of Tobacco.Thus, findings substantiate that Nicotiana benthamiana is also a major source of the virus inoculum and served as potential alternate host of the disease during the off season.